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In situ structuring of virioplankton through bacterial exoenzymatic activity: interaction with phytoplankton ArchiMer
Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene.
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using...
Tipo: Text Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf
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Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence ArchiMer
Ory, Pascaline; Bonnet, Antoine; Mondeguer, Florence; Breitwieser, Marine; Dubillot, Emmanuel; Graber, Marianne.
Mimachlamys varia is a sub-littoral bivalve encountered from Norway to the Mediterranean Sea, which lives mostly byssally attached to rocks. During the low tide period, M. varia individuals, located highest on the shore, may experience short time of aerial exposure and face a low availability of oxygen. Here we report a comparative metabolomic profiling of gill samples of M. varia obtained by both LC-QToF and APGC-QToF mass spectrometry, to analyze metabolic changes occurring during emersion in comparison with immersion. Scallops were grown in aquaria with a simulated intertidal environment mimicking short-duration air exposure that they might experience during extreme tides: alternating 2 h emersion and 10 h immersion. Our results show a switch from...
Tipo: Text Palavras-chave: Anaerobic; APGC-QToF mass spectrometry; Hypoxia; Intertidal; LC-QToF mass spectrometry; Metabolomics; Mimachlamys varia; PLS-DA.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00466/57741/59936.pdf
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Microbial interactions in marine water amended by eroded benthic biofilm: A case study from an intertidal mudflat ArchiMer
Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J..
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (< 0.6 μ- and < 10 μ filtrates) were diluted either with < 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with < 30 kDa-benthic compounds (BC). The kinetics of heterotrophic...
Tipo: Text Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf
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